Galectin-8 role in Trypanosoma cruzi experimental infection

LEGUIZAMON MS

Congreso; XXXI Reunión de la Sociedad Argentina deProtozoología; 2019

Galectin-8role in Trypanosoma cruziexperimental infection Bertelli A., San Marco L., PascualeC., Postan M., Aoki P and Leguizamón MS** Institutode Investigaciones Biotecnológicas, UNSAM-CONICET. Galectins(Gals) are animal lectins with high affinity for ß-galactosides viacarbohydrates recognition domains that drives the immune response by severalmechanisms. In particular,Galectin-8 (Gal-8) has been involved in the regulation of both, homeostatic andpathological processes. The role of Gal-8 on the inflammatory response remainscontroversial. To analyze it we infected mice with Trypanosomacruzi, the causal agent of Chagas disease, as model of chronic inflammatorydisease. Infected C57BL/6J (iWT) and Gal-8 deficient mice (iGal-8KO) were analyzed at 4 monthspost-infection. The absence of Gal-8 favorsinflammation development in liver, skeletal muscle and heart during infection,but do not modified fibrosis levels.  Fibrosisdegree follows the inflammation level. An increase of neutrophils andmacrophages M2-type in iGal-8KO was observed in heart, by flow cytometry analysis.Similar values of specific chemoattractans were detected in cardiac lysates andserum samples from both infected mice groups. Neutrophils increase is associatedto the absence of preaparesis mechanism (stimulationof phosphatidylserine surface expression on viable leukocytes that is recognized byphagocytes and then removed) that is dependent ofGal-8 participation.Our study shows the relevanceof Gal-8 as an anti-inflammatory mediator in chronic inflammatory infectiousdisease and the involvement of Gal-8 in preaparesis in vivo for the first time.  <!-- /* Font Definitions */@font-face{font-family:Arial;panose-1:2 11 6 4 2 2 2 2 2 4;mso-font-charset:0;mso-generic-font-family:auto;mso-font-pitch:variable;mso-font-signature:-536859905 -1073711037 9 0 511 0;}@font-face{font-family:"ＭＳ 明朝";mso-font-charset:128;mso-generic-font-family:auto;mso-font-pitch:variable;mso-font-signature:-536870145 1791491579 134217746 0 131231 0;}@font-face{font-family:"Cambria Math";panose-1:2 4 5 3 5 4 6 3 2 4;mso-font-charset:0;mso-generic-font-family:auto;mso-font-pitch:variable;mso-font-signature:-536870145 1107305727 0 0 415 0;}@font-face{font-family:Calibri;panose-1:2 15 5 2 2 2 4 3 2 4;mso-font-charset:0;mso-generic-font-family:auto;mso-font-pitch:variable;mso-font-signature:-536870145 1073786111 1 0 415 0;}@font-face{font-family:Cambria;panose-1:2 4 5 3 5 4 6 3 2 4;mso-font-charset:0;mso-generic-font-family:auto;mso-font-pitch:variable;mso-font-signature:-536870145 1073743103 0 0 415 0;}@font-face{font-family:TimesNewRoman;panose-1:0 0 0 0 0 0 0 0 0 0;mso-font-alt:"ＭＳ 明朝";mso-font-charset:128;mso-generic-font-family:auto;mso-font-format:other;mso-font-pitch:auto;mso-font-signature:3 134676480 16 0 131073 0;} /* Style Definitions */p.MsoNormal, li.MsoNormal, div.MsoNormal{mso-style-unhide:no;mso-style-qformat:yes;mso-style-parent:"";margin:0cm;margin-bottom:.0001pt;mso-pagination:widow-orphan;font-size:12.0pt;font-family:Cambria;mso-ascii-font-family:Cambria;mso-ascii-theme-font:minor-latin;mso-fareast-font-family:"ＭＳ 明朝";mso-fareast-theme-font:minor-fareast;mso-hansi-font-family:Cambria;mso-hansi-theme-font:minor-latin;mso-bidi-font-family:"Times New Roman";mso-bidi-theme-font:minor-bidi;mso-ansi-language:ES-AR;mso-fareast-language:JA;}.MsoChpDefault{mso-style-type:export-only;mso-default-props:yes;font-family:Cambria;mso-ascii-font-family:Cambria;mso-ascii-theme-font:minor-latin;mso-fareast-font-family:"ＭＳ 明朝";mso-fareast-theme-font:minor-fareast;mso-hansi-font-family:Cambria;mso-hansi-theme-font:minor-latin;mso-bidi-font-family:"Times New Roman";mso-bidi-theme-font:minor-bidi;mso-fareast-language:ES;}size:595.0pt 842.0pt;margin:70.85pt 3.0cm 70.85pt 3.0cm;mso-header-margin:35.4pt;mso-footer-margin:35.4pt;mso-paper-source:0;}div.WordSection1{page:WordSection1;}