Cytotoxicity of iron oxide nanoparticles with natural compounds as coating. Comparative study on MC3T-E1 and L929 cells

CARRA, MARIANGELES ; GONZALEZ, ARIEL; GRILLO, CLAUDIA; FAGALI, NATALIA; FERNÁNDEZ LORENZO, MÓNICA

Mar del Plata

Congreso; Reunión Anual de Sociedades de Biociencia 2019; 2019

Magnetite nanoparticles (NP) are proposed for application in diagnostic techniques as RMI, treatments for hyperthermia, tissue ablation or as drug carriers. In this work, NPs were synthesized by electrochemical methods1. Once prepared and dried, the NPs were weighed and mixed with alkaline gallic acid (GA) or green tea extract (GT) solutions, to achieve 1mg/ml. In order to obtain stabilizing coatings NPs were sonicated, and then dialyzed and filtered to ensure sterility. The coated NPs were characterized by DLS, TEM and ATR-FTIR. Its hydrodynamic diameters were 26.01±2.95nm (GA coating) and 52.25±7.43 nm (GT coating). The green tea coating NPs also formed agglomerates with sizes up to 185.0±13.5nm.Viability assays of pre-osteoblast (MC3T3-E1) and fibroblast (L929) cells were made after 24h of incubation with 1/10-1/1000 dilutions of the 1mg/mL suspensions. The cells were stained with acridine orange fluorescent dye and the images were obtained with an epifluorescence microscope. The pictures were analyzed by the ImageJ software and the statistical analysis was performed (99.9% confidence). These assays showed that GT coated NPs didn´t decrease the viability in L929 cells in the whole concentration range analyzed. However, MC3T3-E1 cells showed a significant decrease in viability from 10µg/mL (85.4±10.8%). For GA coated NPs, the viability in L929 cells decrease from 50µg/mL (81.0±11.6%); while MC3T3-E1 cells showed a viability reduction from 20µg/mL (86.9±5.3%). Previous reports suggested that iron oxide NP may induce toxicity in cells via ROS production2. From this study and according to other authors3, it could be concluded that the toxic effects of magnetic iron oxide NP are highly dependent on the type of cell encountered with nanoparticles. Consequently, an appropriate cytotoxicity study requires assays with more than one cell line.