Retinoic acid reduces migration of human breast and cervical cancer cells

MARTÍNEZ AL; VANDERHOEVEN F, ; REDONDO AL; VARGAS ROIG LM; SÁNCHEZ AM; FLAMINI MI

Mendoza

Jornada; 2° Jornadas de estudiantes de Ciencias Exactas y Naturales; 2017

Universidad Nacional de Cuyo

Breast and Cervical cancer are common malignant diseases worldwide. Retinoids are currently being used in clinicaltrials to treat or prevent cancer progression. We have previously demonstrated that long retinoic acid (RA) treatment(10-6M) reduced cellular adhesion and migration in breast cancer cells. In addition, we verified FAK/Paxillin andHSP27 nuclear translocation during 3-6 hours of RA treatment. As a consequence, we speculate that RA reducesbreast cancer cell adhesion by inhibiting the formation of focal adhesion complexes and we hypothesize that FAK iscarried by HSP27. The aims of this work were to verify in T47D breast cancer cells if HSP27 interacts withFAK/Paxillin, determine in HeLa cervical cancer cells if FAK/Paxillin are translocated to the nucleus after RAtreatment and define if RA decreases the migration of HeLa cells. Immunofluorescence, western blot and migrationassays were performed in HeLa 1.3 cells (with HSP27 expression) and HeLa 2.2 cells (without HSP27 expression).Additionally immunoprecipitation was used in T47D cells. We observed that FAK interacts with HSP27 in T47Dcells and this interaction is increased after RA treatment. FAK translocates to the nucleus in HeLa 2.2 after RAtreatment, suggesting that HSP27 is not essential in this process. Furthermore, RA treatment (10-5 M) decreases Hela2.2 cells migration. We concluded that HSP27 has not a crucial role in the inhibition of cancer cells migration andFAK nuclear translocation induced by RA. Also, the highest concentration of RA (10-5 M) reduces cell migration inHeLa 2.2 cell