COX 1 AND COX 2 INHIBITIONS BY A DECOCTION OF ANDEAN SPECIES FROM SAN JUAN PROVINCE, ARGENTINE

LIMA B; COIRINI H; TAPIA A; FERESIN GE

San Luis

Congreso; XXXVII Reunión Científica Anual de la Sociedad de Biología de Cuyo; 2019

Sociedad de Biología de Cuyo

Plants are a source of bioactive metabolites; the ability to act in multiple signalling pathways characteristic of inflammatoryprocesses has been reported. The aim is to obtain alternative drugs with higher potency and lower undesirable effects. The speciesAzorella cryptantha, Oxalis erythrorhiza, Pachylaena atriplicifolia and Tetraglochin ameghinoi that grow in Andes scopes from SanJuan, Argentine, are used to treat illness related to inflammatory processes in traditional medicine. The dry aerial parts were used toprepare decoctions (DC) (10% w/v), and were assayed to inhibit cyclooxygenase enzymes (COX-1 and COX-2). The effect wascalculated the percent inhibition of COX isoforms measured by colorimetric assay (Kit No. 701050, Cayman Chemical), diclofenacsodium and etoricoxib were used as reference inhibitors (1-2 µg/ml). The DCs were evaluated at 18, 36 and 72 µg/ml. RegardingCOX-2, DC from A. cryptantha and O. erythrorhiza were actives with inhibitory values of 82.10 and 86.97% respectively, to aconcentration of the 72 µg/ml. On the other hand, all DCs were not selective to inhibit COX-2, the percentage of inhibition in COX-1was lower. These results showed that A. cryptantha and O. erythrorhiza are consistent with the traditional use of these species inphytotherapic preparations with a potential anti-inflammatory effect, by blocking the COX-2 proinflammatory enzyme pathway.