Changes in plant cell walls structure produced by attack of stinkbug Nezara viridula on developing soybean seeds

FRANCO ARIAS; JORGE ZAVALA; MARINA CIANCIA

Cordoba

Otro; XXII Reunión Argentina XVI Congreso Latinoamericano de Fisiología Vegetal; 2018

CHANGES IN HULL CELL WALLS STRUCTURE PRODUCED BY STINKBUG NEZARA VIRIDULA ATTACK ON DEVELOPING SOYBEAN SEEDSCAMBIOS EN LA ESTRUCTURA DE LAS PAREDES CELULARES DEL TEGUMENTO PRODUCIDOS POR ATAQUE DE LA CHINCHE NEZARA VIRIDULA SOBRE SEMILLAS DE SOJA EN DESARROLLOArias Franco Andrés1,2, Zavala Jorge Alberto3,4, Ciancia Marina1,21Universidad de Buenos Aires, FAUBA, Cátedra de Química de Biomoléculas, Buenos Aires, Argentina2Centro de Investigación de Hidratos de Carbono (CIHIDECAR-CONICET,UBA), Buenos Aires Argentina.3Universidad de Buenos Aires, FAUBA, Cátedra de Bioquímica, Buenos Aires, Argentina4 Instituto de Investigaciones en Biociencias Agrícolas y Ambientales (INBA-CONICET,UBA), Buenos Aires Argentina.Nezara viridula is one the most important pests in soybean crops. Plants respond to insect attack inducing defenses against herbivores, as an increase of salicylic acid. It has been reported that mature soybean carbohydrates make up 35% of soybean (SB) seed. Structural carbohydrates from hulls contain pectins, hemicelluloses (galactomannans and xylans), and cellulose, however, there is no detail study on hull polysaccharides in the immature stage, when Nezara attack takes place. The aim of this work was to determine if stinkbug damage produces changes in cell wall polysaccharides. Hull cell wall polysaccharides from seeds of a commercial cultivar were studied. One untreated (CTR) lot and two different treatments were prepared: (1) stinkbug damaged (BD); (2) pods sprayed with salicylic acid (TSA). Sequential extraction with water, 0.05 M CDTA pH 6, 0.05 M Na2CO3, 1M KOH, and 4M KOH was carried out. The major extracts were those obtained with water, CDTA, and 1M KOH. Analyses showed major differences between treatments in structure of mannans (BD and TSA vs CTR), and non-esterified homogalacturonans (HG) and in the ratio RGI:methyl-esterified HG (BD vs CTR). These results show that stink bug attack produces measurable changes in hull cell wall structure from Glicine max.