Oxidative stress and morphological damage in Chlorella kessleri exposed to glyphosate

ROMERO, D.M; RÍOS DE MOLINA, MC; JUÁREZ A. B.

Mar del Plata, Pcia. Buenos Aires. Argentina

Congreso; XLIII Reunión Anual de la Sociedad Argentina de Investigación en Bioquímica y Biología Molecular; 2007

Sociedad Argentina de Investigación en Bioquímica y Biología Molecular

The herbicide glyphosate toxicity is relatively low but coadyuvantspresent in commercial formulations could increase it. Effects of oneformulation were studied in the green microalga(Chlorophyta), exposed to increasing concentrations of glyphosate(0-70mg/l) and coadyuvant (50%) added to the medium, at 24º ± 1ºCand under continuous light and agitation. After 96 hr, there wereevaluated cellular growth by cell counting in Neubauer chamber;CI50 using the software Probit; the malondialdehyde content(MDA, lipid peroxidation parameter) using the technique ofTBARS; antioxidant defences, measuring catalase and superoxidedismutase (SOD) activities and reduced glutation (GSH) levels. Thecellular morphology was analized by fluorescence microscopy.Glyphosate caused inhibition of cellular growth and the IC50 was54.37 mg/l. The activity of antioxidant enzymes and the GSH/10cells increase in a doses dependent way. The MDA/10 cells contentwas significant higher from 50 mg/l (p