AMNIOTIC MEMBRANE CONDITIONED MEDIUM PROMOTES CELL DEATH IN HEPATOCARCINOMA HEPG2 AND HUH-7 CELLS

LUCIANO PÉREZ; RODRIGO RIEDEL; ANTONIO PÉREZ-PÉREZ; MARIANA JAIME; VICTOR SÁNCHEZ MARGALET; CECILIA VARONE; JULIETA MAYMÓ

Mar del Plata

Congreso; LXIV Reunión Anual de la Sociedad Argentina de Investigación Clínica; 2019

SAIC

Abstract/Resumen: The placenta and fetal membranes haverecently been proposed as an important stem cells source forregenerative medicine. Stem cells derived from amnioticmembrane offer considerable advantages over other stem cellsbecause of the ease of collection, their low immunogenicity andminimal ethical and legal barriers are associated with their use.Epithelial amniotic cells isolated from the amnion expressembryonic stem cells markers and have the ability todifferentiate towards all three germ layers. Not only are amnion-derived stem cells applicable in regenerative medicine, but alsohave antitumoral properties. Hepatic failure is one of the majorcauses of morbidity and mortality and despite the developmentin therapies, hepatocarcinoma rates are high worldwide. A fewstudies have demonstrated the antitumoral effects of theamniotic membrane and their cells but little is known about themolecular and cellular mechanisms involved. The aim of thiswork was to study some aspects of cell death induced by theamniotic membrane conditioned medium (AM-CM) inhepatocarcinoma cells. Previous results showed that AM-CMinhibits proliferation of HepG2 and HuH-7 cells. We haveanalyzed the expression of proapoptotic proteins (Caspase-3,PARP-1) by qRT-PCR and Western blot, in HepG2 and HuH-7 cellstreated with AM-CM. We have also analyzed p53 expression byimmunofluorescence. We found a significant increment inCaspase-3 expression and in cleaved Caspase-3 and PARP-1 -measured by qRT-PCR and Western blot, respectively-, after 24and 72 h of treatment with AM-CM in hepatocarcinoma cells. Wehave also observed that AM-CM significant increase p53 nuclearexpression, measured by immunofluorescence. Finally, wedetermined that AM-CM induced DNA fragmentation after 72 h oftreatment in HepG2 cells. Our results begin positioning amnion-derived stem cells as emerging candidates in anticancer therapy.