INFECCIÓN EXPERIMENTAL DE CORDEROS CON DIFERENTES CEPAS DE Mycobacterium avium subespecie paratuberculosis

VERNA, A.; MUÑOZ, M.; MORENO, O.; GARCÍA PARIENTE, C.; GEIJO, M. V.; REYES, L. E., JUSTE, R. A.; PAOLICHI, F.; ROMANO, M. I.; GARCÍA MARÍN, J. F. Y PÉREZ, V

Universitat de Lleida

Congreso; XXIX Jornadas Científicas. VIII Internacionales de la Sociedad Española de Ovinotecnia y Caprinotecnia; 2004

In order to know the pathogenicity of several Map strains and their ability to infect different animal species, an oral experimental infection has been carried out in 30 lambs, divided in 6 groups, infected as follows: (a) bovine strain, directly isolated from the intestinal mucosa of a clinical case; (b) the same strain after being growth in laboratory media; (c, d) two bovine strains from Argentina, with different genetic pattern; (e) ovine strain purified from the intestinal mucosa; (f) control. Cellular and humoral immune responses were assessed, as well as the presence of Map DNA in blood by PCR, along 5 mpi. At this time, lambs were killed and pathological and microbiological studies performed in tissue samples. All the strains were able to infect the animals but differences in the responses were observed. Lesions induced by the ovine strain were most severe.