Chemical characterization and biological activities of the lyophilized decoction from Baccharis grisebachii

GÓMEZ J; SIMIRGIOTIS MJ; LIMA B; VILLEGAS GABUTTI CM; PAREDES JD; GAMARRA-LUQUES C; BÓRQUEZ J; LUNA L; WENDEL GH; MARIA AO; TAPIA A

MENDOZA

Congreso; XXXVI Reunión Científica Anual de la Sociedad de Biología de Cuyo; 2018

Sociedad de Biología de Cuyo

Chemical characterization and biological activities of the lyophilized decoction from Baccharis grisebachii Gómez J1, Simirgiotis MJ2,3, Lima B1,Villegas Gabutti CM4, Paredes JD4, Gamarra-Luques C5, Bórquez J6, Luna L1, Wendel GH4, Maria AO4, TapiaA1(1)Instituto de Biotecnología-Instituto de Ciencias Básicas- Universidad Nacional de San Juan, San Juan, Argentina. (2)Instituto de Farmacia, Facultad de Ciencias, Universidad Austral de Chile, Valdivia, Chile. (3) Center for Interdisciplinary Studies on the Nervous System (CISNe), Universidad Austral de Chile, Valdivia, Chile. (4)Farmacología, Facultad de Química, Bioquímica y Farmacia, Universidad Nacional de San Luis, San Luis, Argentina. (5)Instituto de Medicina y Biología Experimental de Cuyo (IMBECU), CCT-Mendoza CONICET, Argentina. (6)Facultad de Ciencias Médicas, Universidad Nacional de Cuyo, Mendoza, Argentina. (7)Laboratorio de Productos Naturales, Facultad de Ciencias Básicas, Universidad de Antofagasta, Chile.The decoction from Baccharis grisebachii is used as a digestive, to relieve gastric ulcers, cicatrizing and antiseptic in Argentine. A lyophilized decoction (LD) from the aerial parts was evaluated regarding their anti-ulcer, antioxidant and cytotoxic activities, and UHPLC-MS metabolome fingerprinting. The antioxidant properties were assayed by DPPH, TEAC, FRAP and lipoperoxidation inhibition in erythrocytes methods, and the antibacterial activity according to the Clinical and Laboratory Standards Institute (CLSI) guidelines. The LD showed a moderated free radical scavenging activity in the DPPH assay (IC50 = 106 µg/mL) and inhibited the lipid peroxidation in erythrocytes in a 67%, at 250 µg/mL. At a dose of 750 mg/kg, the LD had the highest gastroprotective effect with a 93% inhibition of damage through a mechanism that involves NO and prostaglandins using the rat model of ethanol-induced gastric damage. On the other hand, LD does not induce cytotoxic changes on human tumor and no-tumor cell lines at concentrations assayed. Thirty-one compounds were detected and identified based on the UHPLC OT-MS including, nine correspond to flavonoids, thirteen to caffeoyl-quinic acid derivatives, three to coumarins and three to other different phenolic compounds. The results support that Baccharis grisebachii decoction is a valuable natural product with the potential to improve human health.