Antifungal and antioxidant activities of extracts and compounds from Gentianella multicaulis collected in San Juan.

LIMA, BEATRIZ; SANCHEZ, MARIANELA; LUNA, LORENA; AGUERO, MARÍA BELEN; ZACCHINO, SUSANA; FILIPPA, E; PALERMO, JORGE; TAPIA, ALEJANDRO; FERESIN, GABRIELA

Mendoza

Congreso; XXVIII Reunión Científica Anual de la Sociedad de Biología de Cuyo; 2010

SOCIEDAD BIOLOGICA DE CUYO

Antifungal and antioxidant activities of extracts and compounds from Gentianella multicaulis collected in San Juan Lima B1, Sánchez M2, Luna L1, Agüero MB1, Zacchino S3, Filippa E4, Palermo JA2, Tapia A1, Feresin GE1. 1IBT-ICB, UNSJ, San Juan,blima@unsj.edu.ar ,2UMYMFOR-FCEyN, UBA, Buenos Aires, 3Farmacognosia, Fac. Cs. Bioq. y Farmac., U.N.Rosario 4IMBIV- FCEFyN, UNC. Córdoba The infusion of the aerial parts of G. Multicaulis (Gentianaceae), locally known as ‘nencia’ is used in San Juan province Argentine, as stomachic and as bitter tonic for digestive and liver problems. The antimicrobial and free radical scavenger activities G. multicaulis extracts are reported. The dermatophytes Microsporum gypseum, Trichophyton mentagrophytes and T. rubrum were moderated inhibited by different extracts (MICs 125-250µg/mL). The extracts presented strong free radical scavenging effect in the DPPH assay (45-93%, at 10μg/mL) and FRAP assay at 200µg/mL. A significant positive correlation between total flavonoids, total phenolics and DPPH assay were observed (Pearson coeficient, R= 0.984 and 0.831 respectively, p < 0.01). The antifungal compounds were three xanthones: demethylbellidifolin, bellidifolin, isobellidifolin with equal MICs=50µg/mL. Demethilbellidifolin presented highest antioxidant activity towards the DPPH and FRAP assay. In addition oleanolic acid, ursolic acid and swerchirin, were isolated. The results indicate that G. multicaucalis is a source of antifungal and antioxidant compounds. Acknowledgement: CICITCA-UNSJ, UNR, PICT-2008-0554, CONICET (BL, LL, MBA, PJA, and GEF.)