Antifungal propolis asociated to San Juan medicinal flora, Argentina.

AGUERO MARÍA BELEN; SVETAZ, LAURA; SANCHEZ, MARIANELA; LIMA, BEATRIZ; ZACCHINO, SUSANA; PALERMO, JORGE; WUNDERLIN, DANIEL; FERESIN, GABRIELA; TAPIA, ALEJANDRO

Mendoza

Congreso; XXVIII Reunión Científica Anual de la Sociedad de Biología de Cuyo; 2010

SOCIEDAD BIOLOGICA DE CUYO

We report the in vitro antifungal activity of propolis extracts from the province of San Juan (Argentina) as well as the identification of their main antifungal compounds and botanical origin.The antifungal activity was determined by the microdilution technique, using reference microorganisms and clinical isolates. All dermatophytes and yeasts tested were strongly inhibited by different propolis extracts and Larrea nitida exudate (MICs between 31.25 and 125 µg/mL). The most susceptible yeast was Cryptococcus neoformans (31.25 µg/mL).The main antifungal compounds were methyl-nordihydroguaiaretic acid (MNDGA) 1, nordihydroguaiaretic acid (NDGA) 2 and the MNDGA derivative 3. Both ligans 1, 2 displayed strong activity against clinical isolates of Candidas spp, Cryptococcus spp, Trichophyton rubrum and T. mentagrophytes (MICs and MFCs between 31.25 and 62.25 µg /mL). Additionally, two epoxylignan were isolated from propolis samples and Larrea nitida exudates, showing moderate antifungal activity. This is the first report matching the chemical profile of L. nitida exudates with their corresponding propolis by HPLCMS, giving strong evidences on the botanical origin of studied propolis from Argentina. We are grateful to UNSJ, ANPCYT-PICT2008-0554,CONICET.(MBA,BL,SL,GEF DAW)