Antifungal propolis asociated to San Juan medicinal flora, Argentina

AGUERO MARÍA BELEN; LIMA B; CABELLO R; WUNDERLIN D; ZACCHINO S; SCHMEDA-HIRSCHMANN G,; FERESIN GE; TAPIA A

Ciudad de la Punta - San Luis. Argentina

Congreso; XXV XXVII II REUNIÓN CIENTÍFICA ANUAL DE LA SOCIEDAD DE BIOLOGÍA DE CUYO; 2009

SOCIEDAD DE BIOLOGÍA DE CUYO

During last five years, we carried out  propolis elaboration associated to native flora of Andean piedemont as Baccharis grisebachii, Larrea nitida and Zuccagnia punctata. Since propolis`s ethyl acetate extract (PEE) from Bauchaceta  district showed antifungal activity againts yeast Candida albicans and Cryptococcus neoformans by agar dilution method. A study was undertaken to isolate and identify the main metabolites means bioguided antifungal and bioutography assays,   percolation on Sephadex LH-20 column and silica column. Assay-guided isolation led to a lignan methyl nordihydroguaiaretic acid (MNDGA), as the main active constituents of the crude drug. wich was identified by nuclear magnetic resonance (NMR) spectra using a Bruker spectrometer, operating at 400 MHz for 1H and 100 MHz for 13C, MS and HPLCMS. Yeast Candida albicans C. tropicalis Cryptococcus neoformans were strongly inhibited by MNDGA with a MIC= 31.2 µg/ml.