Glutathione in the control of the intracellular redox homeostasis of rats with copper overload

RODRIGUEZ, A; BOVERIS, A; LAIRION, F; BOGADO, J; REPETTO, MG; LASSO, M; SORENSEN, M

Mar del Plata

Congreso; Reunión Annual de Sociedades de Biociencia. SAIC.SAFE. SAB. SAP. AACYTAL. NANOMED-ar. HCS; 2019

Sociedad Argentina de Investigación Clínica

Copper (Cu(II)) toxicity is associated with oxidative stress (OS) and the oxidation of biomolecules mediated by the generation of reactive oxygen species, mainly hydroxyl radical and organic hydroperoxides (ROOH). The objective of this work is to assess whether oxidative damage (OD) due to acute toxicity of Cu(II) in rats involves changes in the multiorganic redox homeostasis. Male Sprague Dawley rats (200 g) received 0.9% w/v sodium chloride (controls) or three different doses of Cu(II): 5.0, 6.5 and 7.5mg/kg (ip) as copper sulfate. In homogenates of heart (H), kidney (K), lung (L), liver(Li) and brain (B), OS markers were determined by spectrophotometry (6 h): phospholipid oxidation, measured as the content of thiobarbituric acid reactive substances (TBARS); reduced glutathione (GSH) content; enzymatic activity: superoxide dismutase (SOD), catalase and glutathione transferase (GT). Results indicated that TBARS was increased in all the organs and dose, mainly in H, B and Li with increases higher than 100% in H (p