INVESTIGADORES
LABAS Marisol Daniela
congresos y reuniones científicas
Título:
Application Of The ERIC-PCR Technique As A Tool For Following DNA Damage During The Water Disinfection Process With UVC
Autor/es:
CRISTIANI, MARIANA; FLORES, MARINA J.; BRANDI, RODOLFO J.; TEDESCHI, FABIAN; ZALAZAR, FABIAN; LABAS, MARISOL D.
Lugar:
Natal, Brasil
Reunión:
Conferencia; CIPOA- 4TH IBEROAMERICAN CONFERENCE ON ADVANCED OXIDATION TECHNOLOGIES.; 2019
Institución organizadora:
Universidad Federal de Rio Grande del Norte, Brasil
Resumen:
An interesting and novel concept to apply in the follow-up of the water disinfection process is to do it through the damage produced by the disinfectant agent on sensitive components of microorganisms. In this work the ERIC-PCR technique to monitor DNA damage was used. The experimental development was carried out in a batch reactor, using three intensities of UV-C radiation (10.5, 4.2 and 2.1 mW/cm2). For each sample, a DNA extraction was performed for the evaluation by ERIC-PCR. Semi-quantitative information was obtained on the inactivation process of the agarose gels by densitometries that determined the value of the areas of each DNA fragment generated. A linear correlation was obtained with a high correspondence, between the inactivation index obtained through the microbial counts (INACI) and the DNA damage index (DNADI) that was expressed as DNADI = 0.05726 × INACI. It was concluded that the ERIC PCR is a feasible and adequate tool for the detection, estimation and monitoring of DNA damage in the processes of disinfection of water with UV radiation.