Megakaryocyte-stroma cell interactions: effect on proliferation, proplatelet production, and survival

GOETTE NORA; DISCIANI LUPI AILEN; MARTA RF; RUBIO FERNANDA; HELLER PG; LEV PR

Congreso; LXIV Reunion Anual dela Sociedad Argentina de Investigacion Clinica; 2019

Bone marrow SC provide a proper environment for the different hematologic lineages development. They contribute with cytokines, growth factors and extracellular matrix proteins to the regulation of haematopoietic stem cells growth and differentiation. In order to study in vitro megakaryopoiesis and thrombopoiesis, the incorporation of the different SC will be an attractive model. Our objective was to evaluate the participation of different SC on megakaryopoiesis, thrombopoiesis and MK survival in in vitro culture conditions. CD34 positive progenitors were obtained from umbilical cord blood and cultured in stem spam medium supplemented with thrombopoietin, with or without umbilical cord endothelial cells (HUVEC), mesenchymal stem cells (MSC), skin fibroblasts (SF) (all human) or mouse fibroblast cell line (L929). Biological samples were obtained after informed consent and Ethics Commitee approval. The number of MK (CD61-positive cells) and mature MK (CD42-positive cells) increased in the presence of HUVEC (CD61, p= 0.016) or MSC, although the latter did not reach statistic differences (flow cytometry). L929 inhibited MK precursors proliferation and maturation (p