Immunomodulatory effect on the implementation of an ovalbumin-induced allergic response mediated by a non-viable Cutibacterium acnes suspension in mice

ALMOZNI BRENDA; DIAZ AILEN; MANGONE FRANCO; SOTELO AGUSTINA; CANELLADA ANDREA; CASTRO MARISA

San Miguel de Tucumán

Congreso; LXVII Reunión Anual de la Sociedad Argentina de Inmunología; 2019

Allergic diseases are featured by an exacerbated Th2 immune response characterized by the production of cytokines IL-4, IL-5 and IL-13 and an increased production of IgE. One way to prevent or treat this type of disease is to modulate the Th1/Th2 balance to a dominant Th1 profile.Cutibacterium acnes (C.acnes) is a Gram-positive anaerobic bacillus, which exerts important immunomodulatory effects when is used as heat-killed suspensions. Recent reports demonstrated that C. acnes treatment promotes a Th1 immune response.The aim of this work was to evaluate, in a murine model, the ability of C acnes to regulate this Th2-exaggerated response of ovalbumin (OVA)-induced allergy.BALB/c mice were subcutaneously(sc) injected with lyophilized heat-killed C.acnes (500 μg/animal) on days -7, 7, 14 and 21. The OVA immunization (10 ug OVA + 1 mg alum, 0.25 ml) was performed on days 0, 7 and 21 by sc injection. Blood samples were obtained on day 31 when animals were sacrificed. Control group received only the OVA. IgE, IgG, IgG1 and IgG2a in sera and cytokines in spleen supernatant were determined by ELISA.Upon treatment with C.acnes the following effects were observed: a decrease in specific IgE levels (p