Chemokine and prolactin secretion is modified by Ang II and Ang-(1-7) in decidualized compared to non-decidualized human endometrial stromal cells (T-HESC)

SOTELO AGUSTINA; MANGONE FRANCO; MACCHI ROSARIO; PARRADO CECILIA; SALAVERRY LUCIANA; ALMOZNI BRENDA; CASTRO MARISA; REY-ROLDAN ESTELA; CANELLADA ANDREA

Cuidad Autónoma de Buenos Aires

Congreso; 8th Latin American Symposium on Maternal-Fetal Interaction and Placenta (VIII SLIMP); 2019

International Federation of Placenta Associations

To analyze the effect of Ang II and Ang-(1-7) on the secretory profile of human endometrial stromal cells (T-HESC) decidualized in vitro. T-HESC were incubated or not (control) with dibutiryl-cAMP+medroxyprogesterone acetate (db-cAMP+MPA), with or without estradiol (E2) for 4 or 6 days (d). The peptides (125-500 nM) were added at time 0, or 4d. The participation of Ang II receptors, Ang-(1-7) receptor Mas, and NFAT, was analyzed by preincubation with losartan, PD123319, A-779, and INCA-6, respectively, prior to stimulation with the peptides. Prolactin (PRL), IL-8 and MCP-1 levels in 2 or 6d culture supernatants, were determined by ELISA. Differences were considered statistically significant with p