mTORC1 regulation of glycolytic metabolism in proliferating Sertoli cells (SC)

CENTOLA CL; GORGA A; RINDONE GM; PELLIZZARI EH; CAMBEROS MC; RIERA MF; MERONI SB; GALARDO MN

MAR DEL PLATA

Congreso; LXIV Reunión Anual de la Sociedad Argentina de Investigación Clínica; 2019

Sociedad Argentina de Investigación Clínica

The final number of SC reached during the proliferative periods determines sperm production capacity in adulthood. It is well known that FSH is the major SC mitogen exerting its action by activation of PI3K/Akt/mTORC1 dependent pathway. mTORC1 regulates a wide range of cellular functions, including metabolism and cell proliferation. Proliferating cells seem to rely on aerobic glycolysis in order to support anabolic reactions associated with cell cycle progression. Although mature SC metabolism was thoroughly evaluated, the metabolism regulation in proliferating SC has been neglected. The aim of this study was to analyze whether aerobic glycolysis is regulated by FSH through mTORC1 pathway in proliferating SC. SC obtained from 8-day old rats were maintained under basal conditions (B) or stimulated with FSH in the absence or presence of rapamycin (Rap) -specific mTORC1 inhibitor. Lactate (Lac) -glycolytic flux marker- production and 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase isoform 3 (PFKFB3) -isoenzyme which catalyzes the synthesis of a positive allosteric modulator for 6-phosphofructo-1-kinase (PFK1)- expression were evaluated. Results are expressed as mean±SD of three independent experiments (different letters indicate statistically significant differences, P