Study of Expression of Specific Glycans on Platelet Membrane in Congenital Disroders of Glycosylation Patients.

PAPAZOGLU G.M. ; SALINAS R.; PEREYRA M.I.; CUBILLA M.; MARTINEZ DUNCKER I.; ASTEGGIANO C.G.

Ciudad de Mexico

Congreso; 5th Latin American Congress of Glycobiology and 2nd. Meeting of the Glycoscience in Thematic Network; 2019

Sociedad Latinoamericana de Glicobiologia

Introduction: Congenital Disorders of Glycosylation (CDG) are human genetic diseases due to defects in the pathway of glycoconjugates. Most of these patients present thrombo-hemorragic events. Objective: To contribute to the knowledge of the physiopathological bases associated with thrombo-hemorrhagic alterations in CDG patients. Methodology: The expression of total glycans in platelet membrane we studied in a platelet population (PRP) of normal controls (6) and PRP from a PMM2-CDG. The platelet population was identified by flow cytometry labeling using the anti CD-41-PE antibody. A panel of 10 biotinylated lectins labeled with Streptavidin and conjugated with an Alexa-647 antibody was tested to detect glycans. We compared the value of MFI observed in the control group for each lectin studied in relation to the values obtained in the patient PMM2-CDG, by means of a Student's T test considering a significant difference the lower values p