Hexachlorobenzene as a contributor to tumor progression: effects on a HER2-positive model, the LM3 murine breast cancer cell line

ZÁRATE LV; NICOLA CANDIA AJ; ASAD AS; MIRET NV; PONTILLO CA; CEBALLOS LJ; CHIAPPINI FA; CANDOLFI M; RANDI AS

Congreso; LXIV REUNIÓN ANUAL DE LA SOCIEDAD ARGENTINA DE INVESTIGACIÓN CLÍNICA; 2019

Pesticide exposure is proposed as a risk factor for breast cancer, the most frequent tumor in women worldwide. Polychlorinated biphenyls and organochlorine pesticides such as Hexachlorobenzene (HCB), have been identified as endocrine-disrupting-chemicals, compounds that affect the normal mammary gland development. In breast cancer, Estrogen Receptor β (ERβ) is a favorable prognosis indicator (cell anti-proliferation marker), and is expressed in 60% of triple negative breastcancer cell lines. Vascular endothelial growth factor (VEGF) and Cyclooxygenase-2 (COX-2) are associated in tumor angiogenesis. We previously demonstrated that HCB induces migration and invasion in (ERα-, PR-, HER2-) MDA-MB-231 human breast cancer cells, as well as tumor growth and metastasis in vivo . In the present study, we examined the action of HCB (0.005; 0.05; 0.5 and 5 μM) in (ERα-, PR-, HER2+) LM3 murine breast cancer cells. Our results indicated that HCB increases cell proliferation (clonogenic assay) and migration (wound healing assay) at 0.05 and 5 μM (p