Decidual macrophages and trophoblast cell phenotype and function are regulated by VIP in human first trimester placenta

PAPARINI D, VOTA D, CHOUDHURY RH, CALO G, HAUK V, RAMHORST, APLIN JD, ; PEREZ LEIROS C

Congreso; Reunion Anual de la Sociedad Arg de Inmunologia; 2018

Deep placentation disorders are associated with impaired invasiveness of extravillous trophoblast cells (EVT) and vascular remodelling in a pro-inflammatory microenvironment. The vasoactive intestinal peptide (VIP) has anti-inflammatory, pro-secretory and vasodilating effects. However its role in pregnancy is still unclear. Objectives: Here we studied the role of VIP in human first trimester placenta as a regulator in EVT and dedicual macrophages (dMA) functions. Methods: Explants from human placentas (5-9 weeks) were treated with VIP ±VIP-neutralizing antibody (α-VIP) and outgrowth was measured. VIP expression and spiral artery (spA) remodelling were studied by immunostaining assays. Positive selection with CD14-immunomagnetic beads was used to isolate dMA and culturing ±VIP. Gene expression and protein secretion was studied by qPCR and BioPlex assay, respectively. Tubulogenesis assay was performed in uterine endothelial cells (EC) and silencing VIP with a siRNA in BeWo cell line (Tb).Results: VIP increased IL-10 (115.5±37.4 vs. 80.8±32.4 pg/ml) P