LPS from Porphyromonas gingivalis affects trophoblast cell function and immune-trophoblast interaction

ROMÁN MUÑOZ M, CALO G, VOTA D, MERECH FI, PAPARINI D, RAMHORST R, ; PEREZ LEIROS C; HAUK V

Mar cel Plata

Congreso; LXIII Reunion Anual de la SAIC; 2018

Placentation is associated with trophoblast cell differentiation into invasive phenotypes, activation of matrix metalloproteinases (MMP) as well as trophoblast-immune cell interaction to maintain immune homeostasis. Porphyromonas gingivalis is considered to be an important pathogen of periodontal disease that has been also implicated in adverse pregnancy outcome (APO). However the pathophysiology remains unknown. The aim of this study was to examined the effect of an virulence factor such as lipopolysaccharide from Porphyromonas gingivalis (PGLPS) on trophoblast cell function and trophoblast-immune interaction. Cell migration was studied in the human trophoblastic cell line from 1st trimester Swan-71 by wound healing assays and invasiveness in Matrigel-covered transwells. MMPs, TIMPs and cytokine expression was measured by RTqPCR. Whole blood was obtained from healthy donors and neutrophil purified on Ficoll-Triyosom gradient and Dextran sedimentation. The effect of Swan-71 treated with PGLPS conditioned media (CM) on migration and spontaneous apoptosis of neutrophil was analyzed. PGLPS treatment (10ng/ml) significantly reduced cell invasion with an more than five times increased expression of tissue inhibitor of matrix metalloproteinase -1 and -2 (P