The activation of human brain microvascular endothelial cells by brucella abortus infected glial cells is mediated by IL-1Beta

MIRAGLIA, M. C.; FRANCO, MIRIAM M. COSTA ; RODRIGUEZ, A. M.; BARRIONUEVO P.; DELPINO, M.V.; OLIVEIRA, SERGIO C.; GIAMBARTOLOMEI, G.H.

Mar del Plata

Congreso; LIX Reunión Científica Anual de la Sociedad Argentina de Investigación Clínica- LXII Reunión Anual de la Sociedad argentina de Inmunología; 2014

SAIC-SAI

B. abortus can interact with human brain microvascular endothelial cells (HBMEC) and gain access to the central nervous system consequently eliciting the inflammatory pathology named neurobrucellosis. We have recently demonstrated that although B. abortus can infect HBMEC, the indirect effect of glial cells infected with the bacterium is more effective in activating the endothelium. Both, TNF-α and IL-1β secreted by glial cells were reported to mediate HBMEC activation. Since we have demonstrated that these cytokines were secreted by B. abortus-infected astrocytes and microglia, we decided to investigate their role in activation of HBMEC (cytokine secretion ad expression of adhesion molecules). Supernatants from B. abortus-infected astrocytes and microglia induced the production of IL-6, IL-8 and MCP-1 and up-regulated the expression of ICAM-1 on HBMEC. When HBMEC were stimulated with culture supernatants of B. abortus-infected astrocytes and microglia from CASP-1 and ASC KO mice (unable to produce IL-1β), cytokine production and ICAM-1 expression was completely inhibited (p