Antihemolysis induced by arginine-based surfactants

FAIT, M.E.; HERMET, M.; CLAPÉS, P.; COMELLES, F.; PRIETO, E.; ÁLVAREZ, A.; MORCELLE, S.R.; BAKÁS, L.

Salto

Congreso; Latin American Crosstalk in Biophysics and Physiology; 2015

Latin American Federation of Biophysical Societies

Two novel arginine-based cationic surfactants (Bz-Arg-NHCn; Nα-benzoyl-arginine decylamide, n=9, and Nα-benzoyl-arginine dodecylamide, n=11) were synthesized in our Laboratory using as biocatalyst papain, an endopeptidase from Carica papaya latex, adsorbed onto polyamide. Bz-Arg-NHC10 and Bz-Arg-NHC12 were less eye-irritating than the commercial cationic surfactant cetrimide. This knowledge, together with a deeper study of the interaction properties of these compounds with skin would make the two novel arginine compounds a promising alternative to commercial cationic surfactants, especially for their use as penetration enhancers in topical formulations. In this paper, the surface active parameters were studied including CMC (critical micellar concentration) which values were 0.23 and 0.085 µM for Bz-Arg-NHC10 and Bz-Arg-NHC12 respectively. The effectiveness of adsorption is related to the interfacial area occupied by the surfactant molecule Amin were 54.8 and 47 Å2. In this paper we have utilized the erythrocyte membrane as a simplified model system to study the interaction of surfactants with biological membranes. We used sheep (SRBC), and human (HRBC) erythrocytes due to their differences in protein and lipid composition. The hemolytic activity of the three surfactants tested was in the same order in both, HRBC and SRBC, being CT the most potent, follow by Bz-Arg-NHC12 and Bz-Arg-NHC10. All compounds under investigation protected erythrocytes against hypotonic hemolysis. Bz-Arg-NHCn had a wide range of protective concentrations when HRBC are tested, while for SRBC the degree of protection of both surfactant observed is lower than for HBRC and rapidly increased the hemolysis rate beyond their cAHmax values. The antihemolytic effect is a complex process in which the structure of the amphiphile and the specific composition of the membrane play a crucial role.