THE ROLE OF MINTHOSTACHYS VERTICILLATA ESSENTIAL OIL IN THE IMMUNE RESPONSE AND ITS POSSIBLE APPLICATION FOR THE PREVENTION OF BOVINE INTRAMAMMARY INFECTIONS

MONTIRONI, IVANA; REINOSO, ELINA B.; MARÍA INÉS SIRI; PIANZZOLA MARÍA JULIA; MOLIVA, MELINA V.; CAMPRA, NOELIA; RAVIOLO, JOSÉ MARÍA; CARIDDI, LAURA N.

Mar del Plata

Congreso; Congreso SAIC- SAI-SAFIS 2018; 2018

In a previous study we have demonstrated that Minthostachys verticillata essential oil (EO) modulate the pro-inflammatory cytokines expression in a mouse mastitis model at 72 and 96 h post-inoculation (p.i). The aim of this work was to determine the effect of EO on TLR2, TNF- and IL-1expression in a mouse mastitis model challenged with Enterococcus faecium at 24 and 48 h p.i. In addition, antibody production was evaluated at 360 h. Female Balb/c lactating mice were inoculated in mammary glands (MG) as follows: Group 1 (control): 100 µl of PBS/DMSO; Group 2: 100 μl of Enterococcus faecium (EF) (1x108CFU/ml); Group 3: pretreatment with EO and challenge with EF. Gene expression levels were evaluated in the MG by quantitative Real Time-PCR and EF-specific antibodies (IgM, IgG, IgA) in serum and MG homogenates by indirect ELISA. TLR2 and TNF-expression were not detected in treated groups at the times assayed. No IL-1 expression was detected in group 2 at 24 h p.i., however, this expression was increased respect to data obtained in group 1 at 48 h p.i (p