B. PARAPERTUSSIS SUBVERTS IFN-Γ-INDUCED BACTERICIDAL ACTIVITY IN HUMAN NEUTROPHILS

VALDEZ, HUGO; GORGOJO, JUAN PABLO; BAROLI, CARLOS; RODRIGUEZ, MARÍA EUGENIA

Mar del Plata

Congreso; Reunión Conjunta de SAIC SAI SAFIS - LXVI Reunión Anual de la Sociedad Argentina de Inmunología (SAI); 2018

Sociedad Argentina de Inmunologia

Whooping cough is a human re-emerging disease caused by Bordetella pertussis (Bp) and Bordetella parapertussis (Bpp). Its resurgence is partially due to an increase in the incidence of Bpp that started with the introduction of acellular vaccine. This vaccine fails to induce opsonic antibodies against Bpp. Our studies showed that in the absence of opsonic antibodies Bpp survives the encounter with resting neutrophils and macrophages by inhibiting cellular bactericidal mechanisms. The presence of IFN-γ in the in vivo infection environment might change the outcome of these interactions. In this study we investigated the eventual role of IFN-γ in the interaction of Bpp with human neutrophils. To this end, neutrophils were treated or not with IFN-γ. Bacterial phagocytosis and intracellular survival were assayed by fluorescence microscopy and polymixin B protection assays, respectively. Statistical differences were analyzed by ANOVA (p