Chronic administration effects on motor behavioral activity assessed by bioinformatic tools on different stages of a neurodegenerative process in adult male rats.

M P BONACCORSO MARINELLI; FE NIETO GRIMALT; MJ LEDESMA; RJ CABRERA

Buenos Aires

Congreso; LXI reunión anual de la sociedad de investigación clínica; 2016

The brain of patients suffering from Parkinson Disease (PD) shows a profound deficit in dopamine levels, because of the loss of neurons in the nigrostriatal pathway. The progressive neuronal degeneration in substantia nigra pars compacta and the dopaminergical deficit in corpus striatum, generate functional modifications on the basal ganglia system with the consequent characteristic motor dysfunctions such as tremors, stiffness, bradykinesia and gait disorders. It is known that neurosteroids have beneficial properties in the Central Nervous System (CNS), such as protection against noxious agents, enhancenment of cognitive abilities and neurogenesis promotion in human and animal models of neurodegenerative diseases. Because PD affects men more than women, it is believed that female sexual steroids could protect women against this disease.Considering that several reports inform unilateral 6-OHDA lesioned rats as a suitable animal model for behavioral and biochemical evaluation of PD, we use an experimental male rat model of hemiparkinsonism induced by 6-OHDA in which we investigate Estrogen´s role in the functionality of the nigrostriatal dopaminergic system.Particularly in this work, we implement bioinformatic softwares for Open Field Test (OFT) to evaluate locomotor performance before and after a pharmacological induction with amphetamine (AMPH); and for Forced Swimming Test (FST) to asses motor modifications. By using this computerized techniques we´ll be able to early, with high sensitivity and in vivo, infer about estrogen neuroprotective/neuroregulatory actions on PD.