Humoral immune response induced by a Prime-Boost vaccine strategy against recombinant PmpDfrom Chlamydia trachomatis using liposomal formulations with aminoacidicamphiphiles and CpG-ODN as immunostimulants

RUSSI ROMINA CECILIA; REIDEL IVANA; CEPEDA PAULA JULIETA; GRIPPO LUCÍA ; GARCÍA MARÍA INÉS; MULLER DIANA; VEAUTE CAROLINA

Mar del Plata

Congreso; Reunión Conjunta de Biociencias 2018; 2018

Sociedad Argentina de Inmunología

Chlamydia trachomatis (Ct) is afrequently sexually transmitted bacterial worldwide. Vaccine development is strongly needed. The selection of antigenand novel adjuvant play a crucial role invaccine effectiveness. Our aim was to evaluate the ability of a prime boost strategy to induce antibodies against Polymorphic membrane protein D (PmpD).A selected fragment of PmpDcontaining B- and T-cell epitopes was expressed in Escherichia coli. The coding sequence was subcloned in a eukaryotic expression vector (pVAX1).Recombinant protein was formulated withcationic liposomes (Lip) with CpGoligodeoxynucleotide (CpG-ODN) or an aminoacidicamphiphile (AA) as immunostimulants.Female Balb/c mice were immunized with a first intradermal dose of nude plasmid. Two protein boosterswere administered, every three weeks, by intranasal and subcutaneous route, simultaneously. Mice were divided in groups receiving: Lip+CpG-ODN+rPmpD, Lip+AA+rPmpD, rPmpD alone, Lip+CpG-ODN or Lip+AA. Mice were bleed before starting the protocol and ten days after the last dose. Sera anti-rPmpD IgG, IgG1 and IgG2a antibodieswere assessed by indirect ELISA (OD450nm, mean±SEM). Liposome suspensions were stablewith a meanparticle size from 209.0to 248.7nm. Anti-rPmpD IgG were higher in Lip+CpG-ODN+rPmpD(0,52±0,16) and Lip+AA+rPmpD(0.41±0.14)immunized mice compared to Lip+CpG-ODN(0,19±0,03) and Lip+AA(0.11±0.01) (p