Adenosine receptor A2a expression in light induced retinal degeneration. (IBRO-FALAN 2015, Abstract Nro. 752)

SOLIÑO M.; LÓPEZ EM.; MARTIGNONE N.; VACOTTO M.; GIRARDI E.; LÓPEZ-COSTA JJ.

Rio de Janeiro

Congreso; IBRO-FALAN Congress 2015; 2015

International Brain Research Organization (IBRO)

Adenosine receptor A2a expression in light induced retinal degeneration. (IBRO-FALAN 2015, Abstract Nro. 752)Soliño, M., López EM., Martignone N., Vacotto M., Girardi E. and López-Costa JJ.Continuous illumination (CI) of rat retina produces photoreceptor degeneration. It resembles retinal degenerative diseases as retinitis pigmentosa and Age related Macular Degeneration (AMD, first cause of acquired blindness in developed countries). A2a receptor (A2aR) has been described in the retina and has been linked to the control of the characteristics of the subretinal space. It was also seen that it plays a role in inflammation, neuronal and trophic factor activity. In recent years, the modulation of A2aR has emerged as an effectiveneuroprotective strategy to treat a wide range of CNS pathologies.In order to shed some light on the processes underlying retinal degenerations and to evaluate a potential therapeutic target, we decided to study A2aR distribution in control (CTL) and illuminated (IL) rat retinas by immunocytochemistry (ICC), Western blot (WB) and RT-PCR. Sprague Dawley rats were submitted to CI (12000 lux) during 1, 2, 5 and 7 days. Its eyes were extracted and processed by ICC. Other two groups of animals had its retinas extracted and processed by RT-PCR or by WB. We used an A2aR rabbit polyclonal antibody (Santa Cruz Int.) as primary antibody. Results were quantified by image analysis using the software Fiji and Image Light Studio. Data was statistically analysed using one-way analysis of variance tests (ANOVA).In the ICC, the average optical density (OD) was measured for the whole retina and each individual layer separately. Control animals showed strong A2aR immunoreactivity (A2a-IR) on the ganglion cell layer (GCL), the inner nuclear layer (INL), the outer plexiform layer (OPL), and the outer portion of the inner plexiform layer (IPL). A weaker staining was observed in the outer nuclear layer (ONL) and the photoreceptor layer (PH). After illumination, there were two significant rises in the average OD, the first at 48 HS and then again at 5 days of illumination (P