ROLE OF THE TOMATO PHOSPHATIDYLINOSITOL-PHOSPHOLIPASE C2 (SLPLC2) IN DEFENSE RESPONSES INDUCED BY THE PHYTOTOXIN BOTRYDIAL FROM BOTRYTIS CINEREA

GONORAZKY AG; ARRUEBARRENA DI PALMA, ANDRES; ANA MARIA LAXALT

Cordoba

Congreso; Reunion anual de Fisiologia Vegetal; 2018

SAFV

Phospholipase C (PLC) is an enzyme involved in theproduction of the second messenger phosphatidic acid (PA). Thetomato [Solanum lycopersicum (Sl)] phosphatidylinositol-phospholipaseC (PI-PLC) gene family is composed of sixmembers, named SlPLC1 to SlPLC6, differentiallyregulated upon biotic stress. We have shown that SlPLC2-silencedplants are less susceptible to the necrotrophic fungus Botrytis cinerea. Botrydial is a phytotoxic sesquiterpene from B. cinerea that triggers plant defenseresponses. We have demonstrated that botrydial induces PA production in tomatocell suspension via PLC/DGK activation, which is required for reactive oxygenspecies (ROS) generation triggered by botrydial. In thiswork we study the role of SlPLC2in the regulation of defense responses induced by botrydial in tomato plants.Treatment of tomato leaves with botrydial triggered an increase of SlPLC2 transcripts, while no changeswere observed in transcript levels of the other SlPLCs. The expression of SlPLC2 was knocked-down by virus-induced genesilencing and plant defense responses were analyzed upon botrydialtreatment. Preliminary results showno changes in early and late ROS production. Nevertheless, SlPLC2 silenced plants developed less necrotic lesions than controlplants upon botrydial treatment. We will present data on plant defensetranscript levels and callose deposition. The biological relevance of thesedata is discussed. <!-- /* Font Definitions */@font-face{font-family:Arial;panose-1:2 11 6 4 2 2 2 2 2 4;mso-font-charset:0;mso-generic-font-family:auto;mso-font-pitch:variable;mso-font-signature:3 0 0 0 1 0;}@font-face{font-family:"Droid Sans Fallback";mso-font-charset:1;mso-generic-font-family:auto;mso-font-pitch:variable;mso-font-signature:0 0 0 0 0 0;} /* Style Definitions */p.MsoNormal, li.MsoNormal, div.MsoNormal{mso-style-parent:"";margin:0cm;margin-bottom:.0001pt;mso-pagination:widow-orphan;font-size:12.0pt;font-family:"Times New Roman";mso-fareast-font-family:"Times New Roman";mso-bidi-font-family:"Times New Roman";mso-ansi-language:ES;mso-fareast-language:ES;}span.apple-converted-space{mso-style-name:apple-converted-space;mso-style-parent:"";}@page Section1{size:612.0pt 792.0pt;margin:72.0pt 90.0pt 72.0pt 90.0pt;mso-header-margin:36.0pt;mso-footer-margin:36.0pt;mso-paper-source:0;}div.Section1{page:Section1;}-->