Effects of metformin on oocyte maturation and cumulus oophorus apoptosis during IVM of porcine COC

LUCHETTI, CG.; LORENZO, MS.; REVILLA, M.; TEPLITZ, G.; YNSAURRALDE, AE.; LOMBARDO DM.

Mar del Plata

Encuentro; Encuentro SAIC-SAI-SAFIS 2018; 2018

SAIC-SAI-SAFIS

The insulin-sensitizing drug metformin has antioxidant and antiapoptotic properties in a variety of models. We have previously shown that metformin added to in vitro maturation (IVM) medium containing insulin- transferrin- selenium (ITS) has antioxidant effects on porcine COC. It has been shown that metformin plus insulin during in vitro culture of porcine embryos increases the blastocyst rate. The aim of this work was to determine if metformin added to IVM medium has antiapoptotic effects and if it has any effect on oocyte maturation rate. Porcine COC were obtained from slaughterhouse ovaries and matured in vitro during 44-46 h in TCM medium supplemented with ITS (group I), metformin (group M), ITS + metformin (group I+M) or nothing (control). COC were treated with hyaluronidase for obtaining cumulus cells. Early and late apoptotic, necrotic and viable cumulus cells were detected by flow cytometry using annexin-V and PI. Oocyte maturation rate was determined using Hoechst under UV microscope. Data were analyzed by Chi square and Fisher exact test for each pair of columns: The group M had lower and the group I had higher number of apoptotic cells than the other groups (p