INVESTIGADORES
CLEMENTE Marina
congresos y reuniones científicas
Título:
DIFFERENTIAL EXPRESSION OF TWO NOVEL KAZAL-TYPE PROTEINASE INHIBITORS IN ARABIDOPISIS THALIANA LEAVES INFECTED BY BOTRYTIS CINEREA
Autor/es:
PARIANI, S.; SANDER, V.; ROSSI, F.; CONTRERAS, M.; PIECKENSTAIN, F.; CLEMENTE, M.
Lugar:
Mar del Plata
Reunión:
Encuentro; VIII Encuentro Latinoamericano y del Caribe de Biotecnologia; 2013
Institución organizadora:
RedBio Argentina
Resumen:
Previously,
we identified two genes from Arabidopsis
thaliana, which encode for two novel kazal-type proteinase inhibitors. These
genes were named AtKP-1 (accession number: AT4G01575) and AtKP-2 (accession
number: AT3G61980). Also, we demonstrated that these genes have differential
tissue expression. Due to proteases are considered potential virulence factors
for phytopathogenic fungi since they improve the chances for a successful
infection, we evaluate the expression pattern of AtKP-1 and AtKP-2 genes during
Botrytis cinerea infection. Leaves of
A. thaliana plants were infected with
6000 spores of B. cinerea. The
transcript levels of both genes were analyzed in local and systemic leaves by qRT-PCR
during 72 h (6, 12, 24, 48 and 72 h). The local response showed that AtKP-1 gene
was strongly expressed at 24 h after inoculation with B. cinerea, while AtKP-2 gene was weakly expressed. Otherwise, the
systemic response showed that the expression levels of AtKP-2 gene were significantly
increased at 24 h, whereas AtKP-1 transcript levels were similar to those
detected in uninfected plants. In conclusion, these genes would be induced by Botrytis, showing a differential expression
pattern suggesting different roles for these genes in the response of the plant
against infection with these fungi.