EFFECTS OF A HIGH FAT DIET ON TEMPORAL PATTERNS OF METABOLIC PARAMETERS

ALFONSO, J; LÓPEZ M,; NAVIGATORE FONZO LS,; ANZULOVICH AC

TUCUMAN

Jornada; ASOCIACION DE BIOLOGIA DE TUCUMAN XXXV JORNADAS CIENTIFICAS; 2018

ASOCIACIÓN DE BIOLOGÍA DE TUCUMÁN

EFFECTS OF A HIGH FAT DIET ON TEMPORAL PATTERNS OF METABOLIC PARAMETERSAlfonso J, Lopez M, Navigatore Fonzo L and Anzulovich A.Laboratorio de Cronobiología, IMIBIO-SL, CONICET-UNSL. E-mail: javieroscaralfonso@gmail.comThe prevalence of obesity has increased dramatically during the last decades, all over the world. The obesity etiology is multifactorial, and includes genetic, environmental and dietary factors, where hypercaloric diets play a central role in the development of the disease. Recent studies have linked metabolic homeostasis with the circadian clock at molecular, physiological and behavioral levels. The objective of this work was to investigate the effects of a high saturated fat diet on daily patterns of metabolic parameters in Holtzman rats. The animals weaned at 21d of age were randomly separated and fed with a normocaloric diet (ND group) and a high saturated fat diet (HFD group) for 12 weeks. Rats were maintained under 12h-light:12h-dark conditions during the treatment period. On the experiment day, they were sacrificed every 6hs and blood samples were collected. Serum glucose, triglycerides, total cholesterol, HDLc and LDLc + VLDLc were determined by colorimetric assays. We found glucose and triglyceride levels do not vary throughout the day in any of the experimental groups. However, HDLc and LDLc+VLDLc cholesterol levels oscillate in antiphase, over a 24h period, in the serum of the ND rats. Twelve weeks of HFD feeding increased circulating total cholesterol and cholesterol associated to the LDL-VLDL lipoprotein fraction at the beginning of the light period, while decreased HDLc levels during the dark phase. Thus, we can conclude that a diet based on saturated fats modifies the daily patterns of lipid parameters, increasing risk-associated LDLc+VLDLc levels at the beginning of the rest period and decreasing safe-related HDLc levels during the activity period, in the rat.