GC-MS ANALYSIS OF THE ALKALOID EXTRACT FROM THE LEAVES OF HIPPEASTRUM ARGENTINUM (PAX) HUNZ.

JAVIER E. ORTIZ; JAUME BASTIDA; ALEJANDRO A. TAPIA; GABRIELA E. FERESIN

MENDOZA

Congreso; XXXVI CONGRESO DE LA SOCIEDAD DE BIOLOGIA DE CUYO; 2018

SOCIEDAD BIOLOGIA DE CUYO

Alzheimer´s disease (AD) is the most common cause of dementia. The main pharmacological treatment comprises the use of acetyl- and butyrylcholinesterase inhibitors. The Amaryllidaceae family is a source of a wide range of cholinesterases inhibitors alkaloids including galantamine, a long-acting, selective, reversible, and competitive acetylcholinesterase inhibitor, approved by FDA in 2001, and currently used for the treatment of mild and moderate AD. As a part of an ongoing study of Argentinean Amaryllidaceae species in search of new bioactive compounds, the alkaloid extract from the leaves of Hippeastrum argentinum. The aim was the characterization and identification of bioactive compounds in the alkaloid extract from the H. argentinum leaves by GC-MS analysis, and comparing the results with those of the bulbs (previously obtained). The extract prepared from dried and crushed leaves. Them, was analyzed by GC-MS, and these compounds were characterized by comparing their mass fragmentation patterns with standard reference spectra in homemade databases. Major alkaloids detected were 7-OH-clivonine, montanine, undulatine y pancracine. The alkaloid GC-MS profile was similar to those of the bulbs excepting the proportion. Thus, the relative alkaloid concentration depending on the plant´s parts can vary in this species. The alkaloid 7-OH-clivonine isolated from H. argentinum is a cholinesterase inhibitor, more abundant in the leaves than the bulbs.