IS ENDOTHELIAL LIPASE A SUPPORTING ACTOR OF LIPOPROTEIN LIPASE? STUDY FROM THE GENE TO THE ACTIVITY IN AN OBESITY MODEL.

MAGALI BARCHUK,; VERONICA MIKSZTOWICZ,; CEVEY AGATA; VALERIA ZAGO; GRACIELA LOPEZ; ROSARIO MECOZZI,; SILVIA MARIA FRIEDMAN, ; NORA GOREN.; ,CELINA MORALES,; LAURA SCHREIER, ; GABRIELA BERG

Mar del Plata

Congreso; Reunión Anual SAIC SAI SAFIS; 2018

SAIC SAI SAFIS

Lipoprotein lipase (LPL) and endothelial lipase (EL) are involvedin lipoproteins metabolism. In insulin-resistance (IR), with visceraladipose tissue (AT) expansion and cardiac steatosis, this enzymesbehavior is altered. Peroxisome Proliferator Activated Receptors(PPARs) and apoproteins (apo)CII and CIII could be partly responsiblefor these alterations. Aim: evaluate LPL and EL mRNA, proteinlevels and activity in AT and heart, association with lipoprotein profileand the role of PPARs and apoCs in an obesity model.Methods: Male Wistar rats were fed with standard diet (Control,n=14)or High Fat Diet (HFD,n=14) during 14 weeks. Glucose, lipoprotein profile and IR markers were measured. Histological studies wereperformed in heart and epididymal AT. LPL and EL mRNA levelswere assessed by RT-qPCR, proteins levels by Western Blot andenzymes activities by radiometric assays. Cardiac and AT PPARsexpression were measured by Western Blot and hepatic apoCsmRNA by RT-qPCR. The study was approved by CICUAL-FFYB(UBA).Results: In HFD, fat depots were observed in hearts, whereas ATpresented higher adipocyte size. In heart and AT, no differenceswere found in EL mRNA levels between groups, while AT LPL mRNAand protein levels were decreased in HFD (p=0.04 and p=0.01,respectively),without differences in heart. In both tissues, EL proteinlevels and activity were increased (p=0.05 and p=0.001,respectively)and inversely associated with decreased LPL activity(heart: R=-0.64,p