M1/M2 POLARIZATION IN EPICARDIAL ADIPOSE TISSUE AND ITS ASSOCIATION WITH MMP-1 IN CORONARY ARTERY DISEASE.

VERONICA MIKSZTOWICZ,; MAGALI BARCHUK, ; ÁGATA CAROLINA CEVEY,; CELINA MORALES; GRACIELA LOPEZ; LAURA SCHREIER; MIGUEL RUBIO; NORA GOREN; GABRIELA BERG

Mar del Plata

Congreso; Reunión Anual SAIC SAI SAFIS; 2018

SAIC SAI SAFIS

Epicardial adipose tissue (EAT) is a visceral AT, surrounding andinfiltrating myocardium and coronary arteries. An increase in EATvolume is directly related to coronary artery disease (CAD). EATexpansion is accompanied by inflammatory cells infiltration, mainlymacrophages. These macrophages could polarize to non-inflammatory(M2) or to pro-inflammatory phenotypes (M1). M1 could leadto metalloproteinases (MMPs) expression, involved in extracellularmatrix degradation. Little is known about macrophages phenotypesand MMP-1 expression in EAT. Objective: to evaluate infiltration andpolarization of macrophages and its association with MMP-1 exprespatients undergoing coronary artery bypass graft (CAD, n=19) orvalve replacement (No CAD, n=10) infiltration of inflammatory cellswas evaluated by immunohistochemistry. Markers of M1 (MCP-1,IL-6 and IL-12) and M2 (IL-10 and TGF-β) were measured by qRTPCR.MMP-1 localization and levels were evaluated by immunohistochemistryand qRT-PCR respectively. Serum lipid profile, glucoseand insulin were assessed. The study was approved by the EthicCommittee of the Hospital de Clínicas, UBA. Results: In comparisonto No CAD, EAT from CAD patients presented macrophages infiltrationand higher MCP-1 levels (p=0.05). MMP-1 was mainly localizedin perivascular connective stroma and in the basement membranesurrounding adipocytes and its levels were higher in CAD (p=0.033).MMP-1 was directly associated with MCP-1 (r= 0.556, p=0.02) andIL-6 (r= 0.723, p=0.001). Conclusion: In EAT from CAD the increaseof MCP-1 and MMP-1 levels could suggest that M1 macrophagesare the predominant phenotype. This could be partially responsiblefor the expansion and inflammation of EAT in CAD. Therefore, itcould be suggested that the most important factor for the inflammatorystate of EAT could be not only the number of infiltrating macrophagesbut also the macrophage polarity.