CONICET | Buscador de Institutos y Recursos Humanos

Yerba Mate (Ilex paraguariensis) is a native South Americantree but Yerba Mate tea is growing in popularity around theworld by biological properties. This plant contains several activephytochemicals which are responsible for its health benefits. One ofthe most abundant polyphenol compounds in Yerba Mate is chlorogenicacid. It has been reported many biological properties for thispolyphenol, including antioxidant, anti-inflammatory, antiviral, andanticancer activities, and may be responsible for the reduced riskof some chronic disease. We previously showed the anti-tumoralproperties of Yerba Mate extract in several experimental systems.The aim of this study was to explore whether chlorogenic acid is themolecule responsible of biological effects observed in Yerba Mateextract. Total polyphenol concentration was measured using Folin-Ciocalteau method, with a mean of 0.03 gallic acid equivalents/mg Yerba Mate. Several classes of chemical constituents as caffeoylderivatives (chlorogenic acid and caffeic acid) and flavonoids (rutin,kempherol and quercetin) were quantified by HPLC. In vitro assayswere performed using tumor cell lines from different localization(CT26, MDA-MB-231, H125, Colo205, SN12C, PC3). Both, YerbaMate extract and chlorogenic acid inhibited cell proliferation in a dose-dependent manner. However, Yerba Mate extract was the mostpotent inhibitor of cell proliferation at a concentration lower than theconcentrations used of chlorogenic acid (ranged IC50= 0.027-0.1 mMfor YM and 0.312-0.75 mM for chlorogenic acid). On the other hand,we demonstrated that both Yerba Mate extract and chlorogenic acidmodulate cell adhesion, migration and the invasive capacity of tumorcells. Our results suggest that the in vitro biological effects of YerbaMate extract are not only due to chlorogenic acid but also could beadditive and synergistic effects among the different bioactive componentspresent in Yerba Mate

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