Antiproliferative activity of blueberry extract against a diverse panel of tumor cell lines

HUMBERTO LAMDAN; LORENA G. CALIGIURI; ROCÍO GARCÍA LÁZARO; NORAILYS LORENZO; DANIEL F. ALONSO; HERNÁN G. FARINA

Buenos Aires

Congreso; LXII Reunión de la Sociedad Argentina de Investigación Clínica; 2017

SAIC

Blueberries have been considered to be one of the fruitswith the highest antioxidant potentials. Among the most prevalentphytochemicals in berries are the anthocyanin, which are responsiblefor much of their antioxidant activity and have been reported tohave positive impact on health and cancer prevention.We previously showed that a hydroalcoholic blueberry extract inhibitsthe proliferation of CT26 and H125 tumor cells. Both, migrationand invasion of CT26 were also modulated by the extract. Inthis work, we reported the production of a new blueberry extractusing a different extraction protocol. Frozen blueberries (samplesof 1 Kg) were first homogenized in 0,5 L of ethanol (96%) using adomestic blender. Anthocyanins and other phenolic compounds inthe homogenates were then extracted at room temperature during96 h, protected from light. The homogenization step was introducedto achieve a more efficient extraction of anthocyanins. This protocolrendered an extract with a high anthocyanins concentration (505,9mg/L), determined by the pH differential method as cyanidin-3-glucosideequivalents. The antiproliferative activity of the new blueberryextract was evaluated against a diverse panel of tumor cell linesof different origin, including Colo 205, CT26, MDA-MB-231, H125,PC-3 and SN12C. For all cell lines, the blueberry extract showeda dose dependent inhibitory effect on cell proliferation, with IC50concentration ranging from 6,1 to 17,6 mg/L (cyanidin-3-glucosideequivalents). These results provide a direction for further researchesabout the antitumoral potential of blueberries.