IIBBA   05544
INSTITUTO DE INVESTIGACIONES BIOQUIMICAS DE BUENOS AIRES
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
SUPINE FALLS IN Ceratitis capitataCORRELATE WITH DISTINCT GENE EXPRESSION PROFILES
Autor/es:
PÉREZ M.M.; QUESADA-ALLUÉ, L.A.; BOCCHICCHIO, P. A.; CAVALIERE-CANDEDO, V.; RABOSSI, A.
Lugar:
Paraná
Reunión:
Congreso; LIV Reunión Anual Sociedad Argentina de Investigación en Bioquímica y Biología Molecular; 2018
Institución organizadora:
SAIB
Resumen:
The peculiar supine behavior of young males of the pest-fly Ceratitis capitata, measured in experimental laboratory conditions, seems to be apresumptive indicator of longevity. The different profiles of supine falls correlate with a number of behavioral and molecular parameters. When during six days-long experiments flies showing frequent supine falls were compared to those showing low frequency of falls (or not falls at all),using an RNA extraction and sequencing approach, significant differences were found in gene expression. Certain stress genes change more than twice their expression; as well as genes related to ataxias in other animals, including vertebrates. Surprisingly, certain glycosyl transferases,lectins and genes related to the innate immune system might change several-fold their expression. Main data were confirmed by RT-PCR and/or protein biochemistry. Data on supine behavior were also correlate with interaction behavior of the Medflies in three-flies experimentallaboratory minimal lek models using a specially designed arena and recording device that allows the analysis of two-hour bouts, dayly during six days. In many experiments, the age of death was recorded to be correlated with other individual parameters within the lek or compared to similar flies in other leks.