IN VITRO STUDY OF THE TRANSCRIPTIONAL REGULATION OF MALIC ENZYME AND CITRATE SYNTHASE GENES IN SOYBEAN SEEDS

PAVLOVIC, T.; GERRARD WHEELER, M. C.; DRINCOVICH, M. F.; ANDREO, C. S.; SAIGO, M.

Córdoba

Congreso; XXXII Reunión Argentina de Fisiología Vegetal; 2018

Sociedad Argentina de Fisiología Vegetal

Protein and oil seed contents are major traits that impact on the soybean economic value. Dry matter partitioning in the whole plant depends on the source strength and sink capacity. Fortunately, this is a variable trait that can be modified, but the metabolic pathways related to reserve accumulation are complex and they are not completely elucidated. In one species more than one route may be responsible for precursor generation in oil and protein biosynthesis. In this regard, a better understanding of these mechanisms is essential for the development of biotechnological tools. Previous studies in our group demonstrated that -NAD and -NADP dependent malic enzymes and citrate synthase are implicated in the provision of carbon and reducing power for fatty acid and amino acid biosynthesis throughout the filling process. The present work is focused on the transcriptional regulation exerted by the Carbon:Nitrogen proportion and ABA presence as possible signals that regulate gene expression during soybean seed development.