P63 DEPLETION REDUCES 3D AND TUMOR GROWTH CAPACITY IN BLADDER CANCER CELLS

MACARENA ZAMBRANO, ; MARIANELA SCIACCA, ; YANINA LANGLE,; DENISE BELGOROSKY, ; EDUARDO IMANOL AGÜERO, ; EDUARDO SANDES,; ANA MARIA EIJAN; CATALINA LODILLINSKY

MAR DEL PLATA

Congreso; Reunión Conjunta de la Sociedad Argentina de Investigación Clínica (SAIC), la Sociedad Argentina de Inmunología (SAI) y la Sociedad Argentina de Fisiología (SAFIS).; 2018

Based in the need to develop more precise therapies, bladder can¬cer has been classified related to its gene expression pattern in lu¬minal like and basal like bladder tumors. The biological and clinical significance of these signatures remains unclear. p63 characterize basal like bladder tumors. Nevertheless the p63-related molecular pathways in this pathology are not well known yet. Our hypothe¬sis is that p63 acts as a protumoral factor, by activation of invasive signaling programs. Our previous results showed that p63 depleted bladder cancer cells have lower extracellular matrix degradation and migratory capacity associated with the lost of MT1-MMP expression.Knocking down for p63 in human bladder cancer cell lines MGHU3 and UMUC14 was developed using a TET-ON system where p63 depletion is induced externally under doxycycline (DOX). Both cell lines were grown in 3D conditions during 30 days treated with o without DOX and spheroids diameter was analyzed. p63 deplet¬ed spheroids (DOX) growth was significantly lower than untreated controls (CRL) (ANOVA with Tukey comparisons, p