COOPERATION BETWEEN EPITHELIAL AND MYOEPITHELIAL CELLS IN BREAST CANCER PROGRESSION

MARIANELA SCIACCA, ; MACARENA ZAMBRANO,; DENISE BELGOROSKY,; YANINA V. LANGLE, ; EDUARDO IMANOL AGÜERO, ; EDUARDO SANDES,; ANA MARIA EIJAN; CATALINA LODILLINSKY, N

MAR DEL PLATA

Congreso; Reunión Conjunta de la Sociedad Argentina de Investigación Clínica (SAIC), la Sociedad Argentina de Inmunología (SAI) y la Sociedad Argentina de Fisiología (SAFIS).; 2018

Normal ductal of mammary gland is lined by two main cell types, the luminal (LEP) facing the lumen and the myoepithelial (MEP) facing the stroma. Most of breast tumors are generated from LEP, but the role of MEP in tumorigenesis is not clear. The cellular model LM38 consists in three cell lines: LM38-LP (epithelial and myoepithelial), LM38-HP (epithelial) and the LM38-D2 (myoepithelial).Previously we demonstrated that only the bi-cellular LM38-LP cell line was able to develop intraductal tumors, suggesting that cell in¬teraction could confer an advantage for tumor formation and pro¬gression. In this work, we examined different cell mechanism like survival, adhesion and invasion.The viability of three cell lines was analyzed by MTS assay after 24 and 48 hours, in different concentrations of bovine serum (SFB). LM38-LP cells had the highest viability with 1% and 5% of SFB com¬pared to LM38-HP and LM38-D2 lines (p