BORON NEUTRON CAPTURE THERAPY (BNCT) COMBINED WITH BCG IMMUNOTHERAPY IN AN ECTO¬PIC COLON CANCER MODE IMMUNOLOGICAL RESPONSE AND CYTOTOXIC EFFECT

VERONICA ANDREA TRIVILLIN ; YANINA LANGLE, ; LUCAS L COLOMBO, ; EMILIANO CC POZZI ; SILVIA I THORP,; MARCELA A GARABALINO, ; ANDREA MONTI HUGHES, ; PAULA CUROTTO, ; PAULA S RAMOS, ; IARA SOFÍA SANTA CRUZ, ; ANA MARIA EIJAN; AMANDA E SCHWINT.

MAR DEL PLATA

Congreso; Reunión Conjunta de la Sociedad Argentina de Investigación Clínica (SAIC), la Sociedad Argentina de Inmunología (SAI) y la Sociedad Argentina de Fisiología (SAFIS).; 2018

BNCT combines selective tumor uptake of 10B compounds and neu¬tron irradiation. The abscopal effect refers to the inhibitory action of radiotherapy on tumor growth at a site distant from the area of irra¬diation. Bacillus Calmette-Guerin (BCG) is known to induce a potent cytotoxic immune response. Having demonstrated the capacity of BNCT alone to induce abscopal effect, the aim of the present study was to evaluate the local therapeutic efficacy of BNCT combined with BCG immunotherapy in the BDIX rat ectopic colon cancer mod¬el and assess the abscopal effect and the tumor specific cytotoxicity. BDIX rats were inoculated with syngeneic colon cancer cells in the right leg. Four weeks post-inoculation, tumor was locally irradiated at RA-3 employing borono-phenyl-alanine (BPA): BNCT-group and BNCT+BCG-group (three intratumoral applications of BCG). BCG-group: BCG only; Beam only-group (BO-group): irradiated without BPA; BO+BCG-group; Sham-group: untreated tumor-bearing ani¬mals (tumor in both legs); Naive: untreated tumor-bearing animals (tumor in left leg). To evaluate abscopal effect, two weeks post-BNCT, colon cancer cell were inoculated in left leg. Once weekly for 7 weeks post BNCT tumor volume was measured in both legs. A significant local therapeutic efficacy was observed in the BNCT-group and BNCT+BCG-group vs. Sham-group p