EFFECT OF VITAMIN K2 SUPPLEMENTATION ON VASCULAR CALCIFICATION IN PATIENTS IN HEMODIALYSIS: A RANDOMIZED CONTROLLED TRIAL

PEREZ ABUD, GRDP; LEON DE LA FUENTE, R; MOYA, A; QUIPILDOR, N; GARCIA BUSTOS, MF; WANGESTEEN FUENTES, R; VAZQUEZ, J; RAMOS, A; SINGH, CDV; MORENO, D; PALOMINO, J; TAMAYO, SR

CABA

Encuentro; REUNIÓN CONJUNTA DE SOCIEDADES DE BIOCIENCIAS; 2017

Sociedad Argentina de Fisiología (SAFIS) y otras

Vascular calcification (VC) is the leading cause of death in patients with chronic renal failure (CRF) on hemodialysis (HD). The vitamin K-dependent Gla protein matrix is one of the most potent inhibitors of VC and in renal patients it is inactive. This risk of mortality is due in part to the development of the calcification of the middle layer. Objective: To evaluate the effect of vitamin K2 on vascular calcification in patients with CRF in HD of the Center of Dialysis Salta. Materials and Methods: prospective, randomized, double-blind study. Inclusion criteria: men and women ≥ 18 years, HD therapy ≥ 6 months, without oral anticoagulants consumption and signed informed consent. The study subjects (n = 58) will constitute a control group: n = 29 (1000 μl of saline solution) or treated group: n = 29 (1000 μl of Vitamin K2). Vitamin K2 will be administered 3 times a week using the same vascular access, at the end of each dialysis session. Blood samples will be drawn for biochemical determinations: Ca, P, PTHi and KPTT. VC will be evaluated before and after 3 months of treatment by carotid artery intima-mediated echocardiography (IME). We performed Shapiro-Wilks test to analyze normality, F-test to compare variances, Student?s t test with Welch modification for normal data with unequal variances and Mann-Whitney test. The statistical package StatGraphics and GraphPad Prism 5 were used. Results: Baseline analysis in patients treated and not treated with Vit K2: Ca 9.2 ± 0.2 vs 9.6 ± 0.5 (p = 0.4); P 5.2 ± 0.2 vs 4.3 ± 0.2 (p = 0.006); PTH 441 ± 104 vs 318 ± 62.8 (p = 0.6). Treated and untreated group: Right IME: 0.63 ± 0.07 vs 1.45 ± 2.1 (p = 0.06). Left IME: 0.73 ± 0.09 vs 0.80 ± 0.1 (p = 0.4). Conclusion: It is expected that vascular calcification does not progress in the treated group when compared with the control group.