Regulation of HO-1 and Sod2 genes in p53 knockout embryonic stem cells

AYELEN TORO; CLAUDIA SOLARI; ARIEL WAISMAN; VICTORIA PETRONE; CAMILA VAZQUEZ ECHEGARAY; MARCOS FRANCIA; SOLEDAD COSENTINO; DIEGO POPRITKIN; ELIZABETH NEW; ELBA VAZQUEZ; ALEJANDRA GUBERMAN

CABA

Simposio; Simposio Fronteras en Biociencia 3; 2018

Embryonic stem cells (ESCs) possess several mechanisms that ensure the genome stability. Reactive oxygen species (ROS) contribute to cellular pathways, including cell proliferation, adhesion and survival. Particularly, their balance in stem cells is not only relevant for differentiation but also to preserve their potential. Heme oxygenase-1 (HO-1) and Superoxide dismutase 2 (Sod2) are two essential antioxidant proteins involved in maintaining redox homeostasis. Besides its function as genome guardian, p53 is involved in ROS generation and some of its target genes, such as HO-1 and Sod2, are modulated by these species. Several reports have studied the relationship between these antioxidant proteins and p53 pathways, however little is known about their association in ESCs. In this work, we aimed to study how the lack of p53 influences the antioxidant defense and the fundamental properties of ESCs.We compared gene expression by RT-qPCR, Western blot (WB) and/or immunofluorescence (IF) of wild type ESCs and a p53-/- ESC line previously generated by CRISPR/Cas9 technology. We found that HO-1 (p