CYTOTOXICITY, ANTIMICROBIAL ACTIVITY AND MORPHOLOGY OF BACTERIAL CELLULOSE WITH CHITOSANE FILM LOADED WITH CIPROFLOXACIN

CACICEDO ML; AMARAL AC; BARUD HS; ISLAN GA; AMARAL CST; PACHECO G; MARTINS GL; DE CARVALHO RA; CASTRO GR

Araraquara

Simposio; II International Symposium of Medicinal Chemistry and Regenerative Medicine; 2017

Universidad de Araraquara

Introduction and objectives: Treatment of skin lesions is a great clinical importance, justifyingthe high investments in new products that reduce the time of healing and increase the patientcomfort. As a result, different products and patches are market, however, most are imported andmake the treatment expensive. In this context, biopolymers gain prominence in the industry dueto its efficient, abundant and low cost, such as bacterial cellulose and chitosan. In the presentwork, the objective was to analyze the cytotoxicity, antimicrobial property and morphologyof the film produced by bacterial cellulose and chitosan associated with ciprofloxacin.Materials and Methods: Cytotoxicity assay was performed by the MTT reduction method({3- (4,5-dimethylthiazol-2-yl) -2,5-diphenyltetrazolium bromide), which allows measuring theamount of viable cells based on the principle reduction of MTT salt by mitochondrial enzymesto formazan (ISO 10993-5), in human fibroblast cells GM07492.Inhibition halos againstPseudomonas aeruginosa and Staphylococcus aureus were determined by using modified diskdiffusion method according to international clinical standards (CLSI/NCCLS), replacing disksfor empty and ciprofloxacin loaded BC/chitosan films in the agar plate surface (Mueller Hintonagar) and inoculated with bacteria (0.5 McFarland scale). SEM experiments were carried out usingsamples previously coated with evaporated carbon. The images were obtained using the JEOLT-300 microscope operating at 2 kV. Results: Cytotoxicity assay demonstrated that the negativecontrol group and CB determined the same pattern of cell viability, evidencing the absence oftoxicity from the extract of the CB membranes analyzed. Ciprofloxacin loaded BC/chitosansamples exhibited a significant but slight decrease in the metabolic activity of cells. In contrast,it does not characterize a cytotoxic influence, considering the percentage of viability exhibitedin the analyzes (greater than 80%).Antimicrobial activity tests, the ciprofloxacin loaded BC/chitosan film demonstrated activity for both bacteria tested, in evidence against Pseudomonasaeruginosa that showed higher activity than against Staphylococcus aureus. The results alsoshowed the antimicrobial activity of chitosan against Pseudomonas aeruginosa, evidenced inthe small inhibition halo formed when inoculated the BC/chitosan film, without ciprofloxacin.Analysis of SEM images revealed surface and transversal section morphology of films. Thesurface was homogeneous and characteristic of chitosan. From the transversal sectional images,was observed the chitosan in the center of film immersed in the bacterial cellulose membrane,and at the extremities of the film. Conclusions: Generally, the film did not prove to be toxic,in addition, it presented antimicrobial activity against the tested microorganisms and had itsmorphological structure characterized. Presented research work will open new prospect for thedevelopment of composites that could be used as wound dressing and them potential applicationsin tissue engineering.