Differential expression of PI-PLC in tomato cells exposed to salt stress

RAMIREZ, L.; HAVE, A. T.; LAMATTINA, L.; ANA MARIA LAXALT

Mar del Plata

Congreso; SAIB; 2007

PL-P67. DIFFERENTIAL EXPRESSION OF PI-PLC IN TOMATO CELLSEXPOSEDTOSALTSTRESS Ramírez L, ten Have A, Lamattina L, IIB, FCEyN, UNMdP, Mar del Plata, Argentina. E-mail: lramirez@mdp.edu.ar PI-PLC Solanum lycopersicum PI-PLC PLC Supported byANPCyT,CONICETandUNMdP. LaxaltA. Salt stress is known to generate phosphatidic acid via the concerted action of phosphoinositide-specific phospholipase C (PI-PLC) and 1,2-diacylglycerol kinase. Both enzymes are encoded by large gene families, e.g. six PI-PLCs genes have been identified in tomato so far. We are interested in elucidating the gene-specific expression profiles of individual during salt stress in tomato ( ). We studied the expression patterns of s by RT-PCR in tomato suspension cells exposed to different doses of NaCl and KCl. The results show a differential expression depending on the dose and the duration of the treatment. Activation of signaling enzymes is often followed by a quick, transient increase in corresponding transcript levels, supposedly in order to replenish enzymes lost by turnover. Thus, the genes that are up-regulated might encode for the enzymes that are involved in salt stress signaling. Nitric Oxide (NO) is a second messenger related to abiotic stress responses in plants. Our laboratory showed that treatments with salt both induced NO production and activated PIPLC signaling in tomato cells. Future analysis will be performed to unravel the role of NO in the activation of PI-PLCs during salt stress and the unequivocal identification of the responsible genes.