EFFECT OF PRENATAL HYPERANDROGENIZATION ON UTERINE TISSUE

SILVANA ROCÍO FERREIRA; ALICIA ALEJANDRA GOYENECHE; GISELLE ADRIANA ABRUZZESE; CARLOS MARCELO TELLERIA; MARÍA FLORENCIA HEBER; ALICIA BEATRIZ MOTTA

BUENOS AIRES

Congreso; Joint Meeting of Bioscience Societies; 2017

SAIC

Abstract: Polycystic Ovarian Syndrome (PCOS) is the most common endocrinopathy at reproductive. PCOS is characterized by oligo or anovulation, hyperandrogenism and ovarian cysts. PCOS involves several health complications such as, insulin resistance and diabetes, obesity, infertility and endometrial cancer. Prenatal hyperandrogenism (PH) is hypothesized as one of the main factors contributing to PCOS. We aim to investigate which parameters are altered by PH in the uterine tissue. Pregnant Sprague Dawley rats were injected with testosterone (1mg) during 16 to 19 days of pregnancy. A control group (C) was generated by vehicle injection (vegetable oil). We define female offspring phenotype as PH ovulatory (PHov) or anovulatory (PHanov). All animals were sacrificed at 90 days of life. The glandular density of the sub-epithelial and stromal compartments were evaluated in histological sections upon H&E staining. The oxidative status was studied by lipid peroxidation and GSH. Androgen receptor (AR) and phosphorylated Akt-Ser473 were assessed by western blot. AR was also studied by immunohistochemistry. Data were analyzed by 1-way ANOVA or Student T-test. Our results showed that PH increased the glandular density of the sub-epithelial and stromal uterine compartments. Lipid peroxidation was significantly increased in the PHanov group when compared with C and PHov groups (p