HIGH FAT DIETS TO OBTAIN AN EXPERIMENTAL MODEL OF OBESITY IN RAT

ALFONSO JO, ; NAVIGATORE FONZO LS, ; LÓPEZ M,; CARGNELUTTI E, ; ANZULOVICH AC

San Luis

Congreso; XXXV Reunión Científica Anual de la Sociedad de Biología de Cuy; 2017

Sociedad de Biología de Cuyo

HIGH FAT DIETS TO OBTAIN AN EXPERIMENTAL MODEL OF OBESITY IN RAT Alfonso JO, Navigatore, Fonzo LS, López M, Cargnelutti E, Anzulovich AC. FQBF, Laboratorio de Cronobiología, IMIBIO-SL,UNSL, CONICET. E-mail: javieroscaralfonso@gmail.comObesity is one of the major risk factors for diabetes, hypertension and atherosclerosis. In order todesign a diet on a chronobiological basis for obesity treatment in the future, we first worked onobtaining an animal experimental model of obesity, as a consequence of a high-fat diet exposure.The objective of this work was to investigate the effects of high unsaturated and saturated fatdiets on different anthropometric parameters in Holtzman rats. The animals weaned at 21d of agewere randomly separated and fed two types of diet for 4 weeks: one group was fed with anormocaloric diet (ND group) and another group with a high unsaturated fat diet (F1D group).From the fifth week, the F1D group received a diet based on high saturated fats (F2D group) for 4more weeks. Rats were maintained under 12hlight:12h-dark conditions. The anthropometricprofile was evaluated according to the following parameters: food intake, body and fat weight,size and body mass index, final weight/initial weight and weight gain/dietary consumptionindexes. Food intake, body weight and height were measured daily. Our results show that ND andF1D groups did not present significant differences in any of the studied parameters. However,modification of the F1D to the F2D diet resulted in significant differences in the final weight/initialweight index as well as in the weight gain/dietary consumption index, in comparison to the ND.Thus, a diet based on saturated fats is more effective for the development of obesity in this animalmodel.