Encapsulation of Resveratrol in nanovesicles derived from renewable sources

GEMMA GUTIÉRREZ-CERVELLÓ; CARMEN PAZOS-MEDINA; NOELIA D. MACHADO; MARIANA A. FERNANDEZ; PABLO GARCÍA-MANRIQUE

Congreso; Resveratrol 2017- Regional Meeting; 2017

Resveratrol (RSV) is a natural polyphenol mainly found in the seeds and skin of grapes, and red wine. It exists in two isomeric structures, but only trans-RSV has potential health benefits in combating diseases, such as cancer, diabetes, neurodegeneration, cardiovascular disorders, inflammation, and other age-related pathologies. However, this great RSV potential is hindered by its poor pharmacokinetic properties, such as low aqueous solubility, low photostability, short biological half-life, and rapid metabolism and elimination. To overcome these limitations, encapsulation of RSV seems to be a good alternative for using this ingredient in food, pharmaceutical and cosmetic industries.Niosomes are vesicles of nanometric size formed by the self-assembly of non-ionic surfactants. As drug delivery systems, they offer important advantages over other encapsulation technologies, such as their low cost, high stability, and biocompatibility.In our study, niosomes were formulated using Tween 80 and Span 80. Both are food grade surfactants derived from sorbitol (sugar) and oleic acid (fatty acid). Dodecanol (fatty alcohol) was used as membrane stabilizer.Niosomes were prepared by the thin film hydration method. The niosomal formulations were evaluated measuring their particle size and encapsulation efficiency (EE). In order to determine EE, free and encapsulated RSV were separated by means of dialysis and gel chromatography.The niosomes mean size was ~227 nm and no considerable differences were found due to the presence of dodecanol. However, EE for niosomes formulated with dodecanol were higher than those achieved in its absence. On the other hand, when gel chromatography was used as free RSV separation method, EE were greater than those obtained using dialysis.The best formulation enhanced 4 times the RSV aqueous solubility. All these results indicated that our niosomes are able to encapsulate RSV and could control RSV release when these nanovesicles are used in food, cosmetic or pharmaceutical applications.